Primary structure of the Neurospora crassa small subunit ribosomal RNA coding region.

The small subunit rlboaomal RNA coding region for Neurospora crassa I s contained within a 2.7 kb Hindlll fragment in the Plasmid pkD002 (TT Subsequent to subclonlng into the single stranded H13 phage mpi8, complete sequences for both s t rands of the coding regions were determined by i n i t i ating the dldeoxynucleotide chain termination sequencing protocols (2) with ollgonucleotlde primers that are complementary to highly conserved regions In eukaryotlc small subunit rRNA genes (3)The Neurospora sequence i s 1795 nucleot ldes in length and Is 87.6 percent homologous with that of Saccharomyces oerevls lae (4) and 96.9 percent s imilar t o Podospora anserlna (Sogln, unpublished da t a ) .